Dose-Response Curve Fitter
Fit IC50 and EC50 for every compound in a dose-response screen from one CSV upload.
What it does
Upload or paste a CSV with concentration, response, and compound ID—or load sample data. Fit every compound from a plate-reader export in one step instead of curve by curve in desktop software.
Pick 4PL or 5PL, let the tool choose the better model, or set relative vs. absolute IC50 with optional plateau constraints. A summary table shows IC50, Hill slope, R², pIC50, EC10, and EC90 with a fit-quality flag per compound. Click any row to review the curve, exclude outliers, and export SVG or PDF figures. Download all results as CSV or a methods PDF for your manuscript. Copy a share link to reopen the analysis; recent runs are saved in your browser.
Why researchers use it
- Fit an entire HTS plate from one CSV upload
- Catch unreliable IC50 when plateaus are missing
- Avoid one-curve-at-a-time workflows in Prism
- Export figures and methods text for manuscripts
- Keep screen data local—no account or install
Best for
- HTS and dose-response screens
- Kinase and receptor inhibitor panels
- Teaching pharmacology without paid licenses
- SAR tables needing pIC50 values
- Sharing analyses with collaborators or reviewers
What makes it different
GraphPad Prism fits curves well but costs hundreds per year and processes one compound at a time. Free web calculators typically handle a single curve with no batch mode or quality checks. R packages like drc automate batch fitting but require scripting and setup.
This tool accepts a multi-compound CSV, grades every fit with plain-English warnings, picks between 4PL and 5PL automatically, and exports CSV results plus vector figures—free, in the browser, with data staying on your machine.
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