Serial Dilution Planner
Generate pipette-ready serial dilution protocols and 96/384-well plate layouts from stock and target concentrations.
What it does
Enter stock and target concentrations in the units you already use—molar, mass per volume, or percent—plus how many dilution steps you need and the final volume per tube. Select which pipettes you have available.
The tool produces a step-by-step protocol: concentration at each step, transfer volume rounded to your pipette resolution, which pipette to use, and diluent volume. It warns when a transfer is below pipette minimums and adds steps when a single dilution would be too steep. Economy mode reduces total reagent use when samples are limited.
Map the series onto a 96- or 384-well plate, copy the protocol table, print a sheet, or share a link that restores the exact plan for a colleague.
Why researchers use it
- Avoid serial dilution math errors
- Get volumes rounded to your pipettes
- Map dilution steps to plate wells automatically
- Share exact protocols via link with colleagues
- Use less reagent when samples are scarce
Best for
- Dose-response and IC50 experiments
- Standard curve preparation
- ELISA and immunoassay dilution series
- qPCR template dilutions
- Antibody or protein serial dilutions
What makes it different
Spreadsheets and generic web calculators often give exact microliter values that do not match any pipette, so you round by hand and risk error. Mobile apps such as LabCalcPro round to pipettes but lack plate maps and are not available on desktop during planning.
This browser tool combines pipette-aware transfer volumes, 96- and 384-well plate layout with CSV or PNG export, and a shareable link—no install or account. Bench mode shows large check-off steps at the hood; economy mode trims volume when reagents are costly.