Standard Curve Calculator
Turn pasted 96-well absorbance into dilution-corrected sample concentrations with assay-aware curve fitting and QC flags.
What it does
Select BCA, Bradford, or sandwich or competitive ELISA, then paste your 96-well absorbance grid from Excel or a plate reader export. Mark which wells are standards, samples, or blanks; enter standard concentrations and each sample's dilution factor.
The tool blank-subtracts, picks the right curve model for your assay, compares linear, quadratic, and 4PL fits side by side, and back-calculates concentrations with the dilution math shown. Replicate CV above 20% and samples outside the standard range are flagged before you report. Exclude bad wells with one click—each exclusion is logged. Copy results to Excel, download CSV, or save a PDF report with the curve, fit parameters, and QC log. Your last five runs stay in the browser for quick reload.
Why researchers use it
- Skip hand-built Excel trendlines and formulas
- Pick assay-appropriate curve models automatically
- Catch forgotten dilution factors before reporting
- Flag high-CV replicates and out-of-range samples
- Export audit-ready reports for lab notebooks
Best for
- BCA and Bradford protein assays
- ELISA concentration back-calculation
- Reanalysis after plate reader export
- QC review before reporting results
- Supplementary figures for papers or ELNs
What makes it different
Excel requires manual layout, model choice, and dilution math—errors often go unnoticed. GraphPad Prism fits curves well but costs hundreds per year and needs you to reformat plate data first. MyAssays handles 4PL online but offers limited QC and export options.
This browser tool accepts a pasted 96-well grid, applies blank subtraction and assay presets, compares models with visible R², flags replicate CV and range problems, and downloads a PDF report with parameters and excluded wells—no install or account.