Cell Seeding Density Calculator
Calculate cells per well and pipette volume for all six plate formats from one hemocytometer count and confluency target.
What it does
Enter your hemocytometer stock concentration, trypan blue viability, and target seeding confluency. Pick a cell line from ~30 presets—HeLa, HEK293T, CHO-K1, and others—or enter custom cells/cm² and doubling time. Results update as you type.
The grid shows cells per well and microliters to pipette for 6-, 12-, 24-, 48-, 96-, and 384-well plates at once, with viability adjustment so dead cells do not skew your volume. When doubling time is set, a banner estimates hours to assay confluency and flags whether you will hit your deadline. Badges warn when volumes exceed well limits or fall below practical pipetting.
Copy a protocol block for Benchling or Notion, export CSV, or print a sheet. Recent runs save in your browser.
Why researchers use it
- Scale seeding across all plate formats in one pass
- Adjust pipette volumes for trypan blue viability
- Skip ATCC lookups with curated cell-line presets
- Estimate hours until assay-ready confluency
- Warn when pipetting volumes exceed well limits
Best for
- Moving experiments between 6-well and 96-well formats
- Planning transfection when confluency timing matters
- Training students on tissue culture calculations
- HEK, CHO, and adherent line assay setup
- 384-well scaling where microliter limits matter
What makes it different
Generic calculators handle one vessel at a time, default to HeLa charts, and skip viability or export. Westlab covers passaging but shows one format per calculation and buries confluency timing. Vendor reference tables rarely match your line.
This browser tool shows all six standard plate formats from one input set, auto-adjusts volume for viability, estimates time to assay confluency with optional deadline checking, and flags pipetting limits on small wells. Copy a protocol block or print a record—no account, no data upload.