PCR Master Mix Calculator
Plan PCR master mixes for multiple assays with pipette volumes, overage, and a bench sheet you can print or paste.
What it does
Enter each PCR recipe: reaction volume, number of reactions, and an overage factor (default 1.1×). List every component—buffer, primers, enzyme, DMSO, probes, or kit master mix—with stock and final concentrations in nM, µM, mM, M, or fold. Per-reaction microliters, totals with overage, and water to fill the tube update as you type.
Add assay tabs when one plate needs several primer pairs; a consolidated list totals shared reagents across assays. Warnings flag impossible dilutions, reagents that exceed reaction volume, and transfers below 0.5 µL with a suggested stock dilution. Save named protocols in your browser, start from SYBR or GoTaq templates, copy results for your ELN, export CSV, print a bench sheet, or share a link that restores the recipe—no account required.
Why researchers use it
- Skip retyping the same weekly qPCR recipe
- Total reagents across multiple assays on one plate
- Catch final concentration above stock before pipetting
- Flag sub-0.5 µL volumes with dilution hints
- Document mixes with printout or ELN paste
Best for
- Routine SYBR or TaqMan qPCR panels
- Genotyping with several primer pairs per plate
- Custom mixes with DMSO, BSA, or probes
- Core facility prep for 96-well plates
- SOPs and ELN records for reproducible PCR setup
What makes it different
Spreadsheets and vendor calculators (Sigma, Qiagen) usually lock you to their kit list, reset when you close the tab, and leave you to retype totals for the bench. CalcBE handles custom components and shareable links but does not save named protocols or combine several assays in one session.
This browser tool accepts any reagent you define, keeps named recipes locally, runs multiple assay tabs with consolidated totals, warns when a transfer is below 0.5 µL, and outputs a printable bench sheet or CSV—without an account.