Free AI-Assisted

ELISA Plate Mapper

Visually assign standards, blanks, controls, and unknowns on a 96-well ELISA plate with auto-fill, validation, and export—no spreadsheet errors.

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Key facts

Key facts about ELISA Plate Mapper
FactValue
Plate format96-well standard layout
Export formatsMatrix CSV, flat LIMS CSV, JSON template, print PDF
Runs in browserYes — local calculation only
Account requiredNo

What it does

Manual ELISA plate maps in spreadsheets are slow and error-prone—a single transposed well can invalidate an entire assay. ELISA Plate Mapper gives bench scientists a color-coded 96-well grid to click or drag-assign well types, auto-generate serial dilution standard curves, and batch-fill unknown samples in duplicate or triplicate.

Configure start concentration, dilution factor, curve points, replicates, and row- or column-major placement—with optional replicate randomization across plate quadrants to reduce spatial bias. Real-time validation warns when blanks are missing, fewer than two standard points are defined, or too many standards sit on perimeter wells. Optionally paste raw OD reader values to calculate replicate %CV and flag groups above 15%.

Export a flat LIMS table or 8×12 matrix CSV compatible with SoftMax Pro and Gen5, save and reload protocol templates as JSON, copy a sequential pipetting guide, or print a bench map with experiment metadata—all client-side in the browser.

Why researchers use it

  • Replace error-prone spreadsheet plate maps with a visual grid
  • Auto-fill 2× (or custom) standard curves with duplicates or triplicates
  • Batch-assign dozens of unknown samples in one click
  • Catch missing blanks and edge-well bias before pipetting
  • Export reader-ready CSV and pipetting checklists for the bench
  • Reuse identical layouts via JSON protocol templates

Best for

  • Sandwich and competitive ELISA plate planning
  • Standard curve layout with serial dilutions
  • Duplicate/triplicate unknown sample assignment
  • Pre-run layout QC before plate reader acquisition
  • Replicate %CV screening from pasted OD values
  • Bench protocol documentation and team template sharing

What makes it different

Generic spreadsheets lack plate-aware visual layout controls and automated validation rules. ELISA Plate Mapper combines 96-well grid mapping, serial dilution curve auto-fill, edge-effect bias checking, and reader-compatible CSV exports in one free browser tool.

Frequently asked questions

Does this tool fit standard curves or calculate sample concentrations?
ELISA Plate Mapper focuses on plate layout planning, validation, and export. It does not perform 4PL/5PL curve fitting—use Absorbance Curve Calculator for concentration back-calculation from OD data.
What validation rules are checked automatically?
The tool warns when no blank wells are assigned, fewer than two distinct standard curve points exist, more than 30% of standard wells are on the plate perimeter, or replicate groups exceed 15% CV when OD values are provided.
Can I export for my plate reader software?
Yes. Export as a flat table (Well, Type, Label, Concentration, OD, %CV) or an 8×12 matrix CSV with sample labels or concentrations per well—formats commonly imported by Gen5, SoftMax Pro, and LIMS pipelines.
Can I use ChatGPT, Cursor, or another AI agent with this tool?
Yes. The workspace includes an in-browser AI assistant for layout planning and validation interpretation. External agents can validate layouts via API & MCP (/tools/developers) using pepkio_elisa-plate-mapper.

Client source code & registry

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Last updated . Pepkio builds free lab calculators alongside bioinformatics CRO services.